The IEF Marker 3–10 is a ready-to-use, unstained protein marker especially developed for IEF applications. It contains 13 purified proteins ranging in pI from 3–10. It works well on horizontal and vertical one-dimensional IEF gels and can be used for both native and denaturing conditions (e.g., 8 M urea, to determine the isoelectric point (pI) of unknown protein samples. Here is a table showing the pIs and origins of the proteins in the IEF Marker, 3–10:
Band no.ProteinOriginpI*9Cytochrome CPig heart10.78Ribonuclease ABovine pancreas9.57 c, m, aLectinLens culinaris8.3, 8.0, 7.86 c, aMyoglobinHorse muscle7.4, 6.95Carbonic anhydraseBovine erythrocytes6.04 c, aB-LactoglobulinBovine milk5.3, 5.23Trypsin inhibitorSoybean4.52Glucose oxidaseAspergillus niger4.21AmyloglucosidaseAspergillus niger3.5*Temperature = 5 degrees C for values given. pI is temperature dependent, i.e. decreases as temperature increases (approximately –0.005 to –0.03 pH units per degree C)
c = cathodic; m = middle; a = anodic